Isothermal Amplification

Alere Technologies are proud to announce that ArrayTube and ArrayStrip are compatible with Recombinase Polymerase Amplification (RPA), the breakthrough isothermal alternative to PCR. RPA uses enzymes to replace thermal cycling, allowing users to amplify their target DNA or RNA in <10 minutes - even from single molecule starting quantities of template. No thermal cycler is required, just a heat block set to 37-40°C

TwistDx’s RPA process employs enzymes, known as recombinases, which are capable of pairing oligonucleotide primers with homologous sequence in duplex DNA. Through this method, DNA synthesis is directed to defined points in a sample DNA. If the target sequence is indeed present, DNA amplification reaction is initiated; no other sample manipulation such as thermal or chemical melting is required. The reaction progresses rapidly and results in specific DNA amplification from just a few target copies to detectable levels typically within 5 - 10 minutes. The entire reaction system is stable as a dried formulation and can be transported safely without refrigeration.

RPA can be used to replace PCR (Polymerase Chain Reaction) in a variety of laboratory applications and end-users can design their own ultra-sensitive assays.

To find out more about RPA and to place an order, please visit www.twistdx.co.uk

HYBRIDIZATION & STAINING

The resulting biotin labelled RPA products are stringently hybridized to a set of highly discriminative probes that are covalently bound onto the microarrays. The biotin labelled DNA bound to the probes on the array is subsequently stained.

DETECTION & ANALYSIS

The arrays are scanned and analyzed by the ArrayMate™ or ATR03™. The reader allows a visualization of the array, analyzes the information and provides a concise result of the test. The presence of a dark spot indicates a successful hybridization. Alere Technologies software measures the signal intensity of each probe.

For further questions please contact us!