The new CarbDetect AS-2 Kit

(item number 247100096)

Challenge

  • The rapid and global emergence of carbapenemase-producing organisms (CPO) is an alarming threat to patient safety and healthcare systems.
  • CPOs are resistant to all beta-lactam antibiotics and they are usually also resistant against other classes of antibiotics.
  • CPOs are most conspicuous in hospitals and frequently cause nosocomial infections. However, they can asymptomatically colonize healthy carriers and this might play a major role in their global spread.
  • Traditional phenotypic tests are generally time consuming and need well trained lab personal. Furthermore, most tests do not allow a detailed molecular identification of the actual carbapenemase gene that is needed for epidemiological and infection control purposes.
  • Therefore, molecular methods are required for a rapid and reliable identification of carbapenemase genes.
  • This could be solved by combining all relevant targets to date in a single test using a multiplex microarray-based assay for detection and identification.

Solution

  • Starting from a clonal CPO culture, the microarray-based CarbDetect AS-2 assay detects and differentiates nearly all carbapenemase genes within 8h (Hand-on Time 2h) (e.g., blaKPC, blaNDM, blaVIM, blaOXA-48).
  • Other relevant ESBL and NSBL genes can also be identified (e.g., blaCTX-M1, blaCTX-M9, blaOXA-1) as well as additional genes associated with aminoglycoside, quinolone, macrolide, sulfonamide and trimethoprim resistance (e.g., aac(6’), mph, qnr, sul, dfr).
  • The CarbDetect AS-2 assay identifies the genes from a given carbapenem resistant strain which phenotypic methods alone cannot provide.
  • The CarbDetect AS-2 assay provides information which help to prevent and trace outbreaks.
  • The CarbDetect AS-2 assay delivers the best molecular tool for monitoring and control of CPOs.

Benefits

  • multiplex approach which replaces several single- and multiplex PCRs that could detect only the most common carbapenemase genes
  • identification of rare carbapenemase genes
  • identification of ESBL and NSBL genes
  • identification of other relevant antibiotic resistance genes
  • identification of the most prevalent pathogens (Escherichia coli, EIEC, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Citrobacter freundii, Shigella spp., Salmonella spp. and Enterobacter spp.)
  • accurate detection of patients colonized with CPOs will help to prevent outbreaks and determine the best infection prevention pathways

Target genes

Carbapenemase genes

blaBIC, blaDIM, blaGES, blaGIM, blaGOB, blaIMI-3 (NmcA), blaIMI-R, blaIMP, blaSIM-1, blaIND, blaKHM, blaKPC, blaNDM, blaPAM-1, blaSFH-1, blaSMB-1, blaSME, blaSPM-1, blaTMB-1, blaVIM, blaVIM-2, blaVIM-7, blaOXA-23-like, blaOXA-40-like, blaOXA-48-like, blaOXA-51, blaOXA-54, blaOXA‑55, blaOXA-58, blaOXA-134/235/284, blaOXA-143/182/253/255, blaOXA-181/232, blaOXA‑214, blaOXA-279, blaOXA-292

ESBL genes

blaCME, blaCTX-M1/15, blaCTX-M2, blaCTX-M8, blaCTX-M9, blaMOX-CMY9, blaPER‑1, blaPER-2, blaSHV (consensus), blaTEM (consensus), blaVEB (consensus), blaOXA-18, blaOXA-45

NSBL genes

blaOXA-1, blaOXA-2,  blaOXA-7, blaOXA-9, blaOXA-10, blaOXA-60

AmpC genes

blaACC, blaACT, blaCMY, blaDHA, blaFOX, blaMOX, blaMIR 

Aminoglycoside resistance genes

aac(3')-Ia, aac(3')-Ib, aac(3')-Ic, aac(3')-Ie, aac(3')-IVa, aac(6')-31, aac(6')-Ib, aac(6')-II, aac(6')-IIa, aac(6')-IIc, aac-aph, aadA1, aadA2, aadA4, aadB, ant2, aphA, armA, grm, npmA, rmtA, rmtB, rmtC,  rmtD, strA, strB

Macrolide resistance genes

mph, mrx

Quinolone resistance genes

qepA, qnrA1, qnrB, qnrC, qnrD, qnrS

Co-trimoxazol (Sulphonamide and Trimethoprim) resistance genes

sul1, sul2, sul3, dfrA1, dfrA12, dfrA13, dfrA14, dfrA15, dfrA17, dfrA19, dfrA5, dfrA7

Additional Species / Genus Specific Markers

Escherichia coli, EIEC, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Citrobacter freundii, Shigella spp., Salmonella spp. and Enterobacter spp.

Publications

Rapid Identification of Carbapenemase Genes in Gram-Negative Bacteria with an Oligonucleotide Microarray based Assay.
Braun SD, Monecke S, Thürmer A, Ruppelt A, Makarewicz O, Pletz M, Reissig A, Slickers P, Ehricht R PLOS one, 2014

Fast DNA-serotyping of Escherichia coli by oligonucleotide microarray.
Ballmer K, Korczak BM, Kuhnert P, Slickers P, Ehricht R, Hachler H
Journal of Clinical Microbiology, 2006

Carbapenemase-producing Enterobacteriaceae: a two-year surveillance in a hospital in IaÅŸi, Romania.
Sascha D. Braun, Olivia Simona Dorneanu, Teodora Vremeră, Annett Reißig, Stefan Monecke, Ralf Ehricht
Future Medicine, 2016

Genotyping Service

Please send either a clonal culture (e.g., suitable agar plate, stab culture or swab) or a high quality DNA preparation into our lab. We do the genotyping and you get a result file in electronic form. On request, we will perform a phenotypical characterization of antibiotic susceptibility and/or species identification by a VITEK-2 System (bioMérieux Deutschland GmbH).

Array-on-demand

Alere Technologies provides customised manufacturing of microarrays mounted in ArrayTube, ArrayTube2 and/or ArrayStrip. You decide which markers/genes you focus on. It is possible to adapt the existing assays/gene lists according to your requirements!


For further questions please contact us!