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ShigaToxType AS-2 Kit
(item number 245900096)
Identify the Shiga toxin 1 and 2 subtypes
The assay allows rapid, economic and standardized DNA-based subtyping of all known Shiga toxin subtypes (Stx variants classified according to the nomenclature of Scheutz et al. 2012) directly from colony material in a short time. Furthermore, the identification of both, single and multiple Stx subtypes within a clonal isolate is feasible.
Family, genus and species controls
Family, genus and species specific markers were included to identify Enterobacteriaceae (Shigella spp. / EIEC or Escherichia coli) and distinguish them from other species which might contaminate the sample (Citrobacter freundii/braakii, Salmonella spp., Pseudomonas aeruginosa, Klebsiella pneumoniae and Acinetobacter baumannii).
Development of a Rapid Microarray-Based DNA Subtyping Assay for the Alleles of the Shiga toxins 1 and 2 of Escherichia coli.
Geue L, Stieber B, Monecke S, Engelmann I, Gunzer F, Slickers P, Braun SD, Ehricht R.(2014) J Clin Microbiol. Jun 4. pii: JCM.01048-14.
Multicenter evaluation of a sequence-based protocol for subtyping Shiga toxins and standardizing Stx nomenclature.
Scheutz F, Teel LD, Beutin L, PiÃ©rard D, Buvens G, Karch H, Mellmann A, Caprioli A, Tozzoli R, Morabito S, Strockbine NA, Melton-Celsa AR, Sanchez M, Persson S, O'Brien AD. (2012) J Clin Microbiol. Sep;50(9):2951-63.
Alere Technologies provides customised manufacturing of microarrays mounted in ArrayTube, ArrayTube2 and/or ArrayStrip. You decide which markers/genes you focus on. It is possible to adapt the existing assays/gene lists according to your requirements!
Please send either a clonal culture (e.g., suitable agar plate, stab culture or swab) or a high quality DNA preparation into our lab. We do the genotyping and you get a result file in electronic form.
For further questions please contact us!